Drug perturbation gene set enrichment analysis (dpGSEA): a
2021.1.12 Taking advantage of available knowledge, we propose a transcriptomically-driven drug screening approach that utilizes enrichment methods to determine candidate
Charlar en LíneaHigh-content CRISPR screening Nature Reviews Methods
2022.2.10 This Primer by Bock et al. describes the main concepts of CRISPR screening and gives examples of its application as a method for biological discovery, with
Charlar en LíneaDiagnostics Free Full-Text Target Enrichment
2022.6.24 This review summarizes the basic principles of target enrichment utilized by these methods, their multiple variations that have evolved over time, automation approaches, overall comparison of their
Charlar en LíneaIsolation and enrichment of circulating biomarkers for cancer screening ...
2015.11.10 Much research has been performed over the past several decades in an attempt to conquer cancer. Tissue biopsy is the conventional method for gathering
Charlar en LíneaScreening of Biosurfactants SpringerLink
First Online: 01 October 2021 382 Accesses Abstract The development of various screening approaches which are rapid, as well as reliable, and selection of a population of
Charlar en LíneaUsing macromolecular electron densities to improve the enrichment
2023.8.22 We measured both enrichment and diversity because virtual screening methods should identify a variety of scaffolds in addition to a large number of active
Charlar en LíneaAn enrichment method to increase cell-free fetal DNA
2019.4.11 An enrichment method to increase cell-free fetal DNA fraction and significantly reduce false negatives and test failures for non-invasive prenatal screening:
Charlar en LíneaAbsolute binding free energy calculations improve enrichment
2022.8.10 We determined the effectiveness of absolute binding free energy (ABFE) calculations to refine the selection of active compounds in virtual compound screening, a
Charlar en LíneaHigh Throughput Screening Methods for ω
2017.8.27 efficient screening technique. 3. Enrichment Based High Throughput Screening Method Microorganisms constitute an abundant repository of enzymes due to
Charlar en LíneaDNA enrichment and tagmentation method for
2019.10.10 Łukasz Krych et al. uses MinION portable sequencing platform to develop a fast and cost-effective method for bacterial DNA enrichment and tagmentation(ON-rep-seq). Their method allows ...
Charlar en LíneaMicrobial-enrichment method enables high-throughput
2023.10.12 This work introduces microbial-enrichment methodology (MEM) that enables removal of host DNA in human intestinal biopsies and characterization of low-abundance microbial taxa down to 1%.
Charlar en LíneaIJMS Free Full-Text Enzymatic Methods for
2022.11.27 Enzymatic-based mutation enrichment methods have evolved through the years from the traditional approaches using restriction endonucleases that detect a minority of mutations to newer methods
Charlar en LíneaImproved method of structure-based virtual screening based
1. Introduction Virtual screening (VS) is a computational approach used to identify active compounds by predicting their activity. In recent years, it has become a successful alternative and complementary technique to experimental high-throughput screening technologies for drug design, because of its ability to decrease the cost and increase the
Charlar en LíneaDetection and identification of transgenic events by next
2019.10.30 Comparison of commercially available target enrichment methods for next-generation sequencing. J. Biomol. Tech. 24, 73 (2013). Article CAS Google Scholar Meienberg, J. et al. New insights into the ...
Charlar en LíneaHigh-throughput fetal fraction amplification increases
2020.11.15 The percentage of a maternal cell-free DNA (cfDNA) sample that is fetal-derived (the fetal fraction; FF) is a key driver of the sensitivity and specificity of noninvasive prenatal screening (NIPS).
Charlar en LíneaA roadmap for metagenomic enzyme discovery - RSC
2021.1.31 PCR-based screening methods are highly-sensitive and throughput can be enhanced through the use of pooling and deconvolution strategies. 42,43 Amplicon-based analysis of common biosynthetic markers including adenylation and ketosynthase ... Targeted enrichment methods for sequencing can be especially useful where longer
Charlar en LíneaBest Practices for Docking-Based Virtual Screening
2021.1.1 Abstract. Docking-based virtual screening (DBVS) is well placed in modern drug discovery and is widely applied with many success cases by both pharmaceutical companies and academic groups. The recent advances in scoring functions, search algorithms, consensus scoring, protein flexibility and enrichment represent a new era of
Charlar en LíneaAn Appraisal of Bacteriophage Isolation Techniques from
2021.6.17 The main limitations of chemical adsorption methods for bacteriophage isolation are cost of filters and pH sensitivity [146, 152].There also is the disadvantage of filters getting clogged and the non-suitability of the method for marine water [].However, such non-enrichment methods are useful in unbiased concentration of bacteriophages
Charlar en LíneaMulti-layer solid-phase extraction and evaporation—enrichment methods ...
2018.2.12 A combination of both methods enables the enrichment of 22 of the 26 model analytes with a mean recovery of 85% (utilizing the most suitable method for each analyte). A target screening comparison of both methods with each other and an HLB-SPE method shows clear advantages for the evaporation and mlSPE methods in the
Charlar en LíneaAbsolute binding free energy calculations improve enrichment
2022.8.10 We determined the effectiveness of absolute binding free energy (ABFE) calculations to refine the selection of active compounds in virtual compound screening, a setting where the more commonly ...
Charlar en LíneaDouble emulsions as a high-throughput enrichment and
2023.5.9 Developing, high-throughput enrichment methods like that do not favor fast-growing microorganisms and are compatible with commonly-available FACS equipment might serve as a novel approach to ...
Charlar en LíneaEnergies Free Full-Text Data Enrichment as a Method of
2023.2.21 The proposed data enrichment method takes full advantage of multiple commercially available weather prediction sources and the physical continuity nature of wind. It can cooperate with any existing forecasting models that have weather prediction data as inputs. The controlled experiments on three actual individual wind farms have
Charlar en LíneaOptimized Glycopeptide Enrichment Method–It Is All About
2022.6.28 Protein glycosylation is a family of posttranslational modifications that play a crucial role in many biological pathways and diseases. The enrichment and analysis of such a diverse family of modifications are very challenging because of the number of possible glycan–peptide combinations. Among the methods used for the enrichment of
Charlar en LíneaHigh throughput, label-free isolation of circulating tumor cell ...
2022.6.13 Even when screening whole blood at a throughput of 100 mL/h, the Cluster-Wells is more sensitive than every CTC cluster enrichment method, only missing ~10.4% of doublets and ~2.7% of triplets.
Charlar en LíneaCancers Free Full-Text Pre-PCR Mutation-Enrichment Methods
2022.5.30 This review describes the available pre-PCR enrichment methods and focuses on the most recently developed techniques (NaME-PrO, UVME, and DEASH/MAESTRO), emphasizing their applications in liquid biopsies. ... The data indicate that the combination of ND-NaME-PrO with HRM can be a practical pre-screening test to
Charlar en Línea(PDF) An enrichment method to increase cell-free fetal
2019.4.11 An enrichment method to increase cell-free fetal DNA fraction and significantly reduce false negatives and test failures for non-invasive prenatal screening: A feasibility study April 2019 Journal ...
Charlar en LíneaTarget Enrichment Approaches for Next-Generation
2022.6.24 Target Enrichment Approaches for NGS. The underlying principles of the two prominent target enrichment technologies are depicted schematically. ( A) The PCR amplicon-based method employs PCR amplification using sequence-specific, target-spanning primers to amplify and enrich the genomic regions of interest (targets).
Charlar en LíneaExpanding the effectiveness of screening Nature Chemistry
2021.6.1 Improvements to enhance the screening of DNA-encoded libraries and give more accurate outcomes than the current one-dimensional affinity-based assays or to expand the chemical space covered by DNA ...
Charlar en LíneaSelection and screening strategies in directed evolution to
2019.12.27 The combination of the two pressures led to prominent enrichment of more stable proteins. mRNA display/cDNA display. First proposed in 1997, mRNA display is a screening method based on the formation of a binary complex between a protein and its encoding nucleic acid in vitro (Nemoto et al. 1997; ...
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